Description
This detailed volume collects chapters that seek to expand our knowledge of molecular events and extended molecular networks in gene regulation. The contents explore numerous aspects of miRNA biogenesis, a sophisticated series of events that assure a finely tuned regulation of miRNA expression and activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, miRNA Biogenesis: Methods and Protocols aims to contribute to the further development of knowledge and application of miRNA biogenesis, both in the basic research lab and in the clinic. Chapter 1 microRNA biogenesis Review Chapter 2 Identifying microRNA transcription sites Chapter 3 Annotating primary microRNAs Chapter 4 Defining Microprocessor co-factors Chapter 5 Endogenous assay for primary microRNA processing Chapter 6 In vitro assay for primary microRNA processing Chapter 7 miRtrons Chapter 8 Inhibiting primary microRNA processing Chapter 9 Precursor microRNA characterization Chapter 10 Precursor microRNA export Chapter 11 Processing of precursor microRNAs in the cytoplasm Chapter 12 Composition of the RNA-induced Silencing Complex Chapter 13 RNA-sequencing of mature miRNAs Chapter 14 qPCR measurement of mature microRNAs Chapter 15 Stability of mature microRNAs Chapter 16 MicroRNA modifications Chapter 17 MicroRNA tailing and trimming Chapter 18 RNA modifications in microRNA biogenesis Chapter 19 MicroRNA biogenesis in disease Case study
