Imaging and Tracking Stem Cells: Methods and Protocols (Methods in Molecular Biology)

Imaging and Tracking Stem Cells: Methods and Protocols gathers representative protocols related to the vital techniques of stem cell imaging and lineage tracing, including that of live cells, both in vivo and in vitro. The detailed chapters presented within have been validated for reproducibility and are described in an easy to follow, step-by-step fashion so as to be valuable for not only experts but also novices in the stem cell field.  As with other volumes in the highly successful Methods in Molecular Biology series, chapters conclude with a Notes section, which provides tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Imaging and Tracking Stem Cells: Methods and Protocols provides both a flavor of the field as it currently is and a source to stimulate new approaches and methodologies by those interested in tracking stem cells and their progeny. 1. Primary Culture and Live-Imaging of Adult Neural Stem Cells and Their Progeny             Felipe Ortega, Benedikt Berninger, and Marcos R. Costa   2. Labeling and Tracking of Human Mesenchymal Stem Cells Using Near-Infrared Technology             Marie-Therese Armentero, Patrizia Bossolasco, and Lidia Cova   3. High Content Imaging and Analysis of Pluripotent Stem Cell-Derived Cardiomyocytes             Gbor Fldes and Maxime Mioulane   4. A High Content Imaging-Based Approach for Classifying Cellular Phenotypes             Joseph J. Kim, Sebastin L. Vega, and Prabhas V. Moghe   5. Conversion of Primordial Germ Cells to Pluripotent Stem Cells: Methods for Cell Tracking and Culture Conditions             Go Nagamatsu and Toshio Suda   6. Imaging and Tracking of Bone-Marrow-Derived Immune and Stem Cells             Youbo Zhao, Andrew J. Bower, Benedikt W. Graf, Marni D. Boppart, and Stephen A. Boppart   7. Co-Visualization of Methylcytosine, Global DNA, and Protein Biomarkers for In Situ 3-D DNA Methylation Phenotyping of Stem Cells             Jian Tajbakhsh   8. Noninvasive Imaging of Myocardial Blood Flow Recovery in Response to Stem Cell Intervention             HuaLei Zhang and Rong Zhou   9. Live Imaging of Early Mouse Embryos Using Fluorescently Labeled Transgenic Mice             Takaya Abe, Shinichi Aizawa, and Toshihiko Fujimori   10. Live Imaging, Identifying, and Tracking Single Cells in Complex Populations In Vivo and Ex Vivo             Minjung Kang, Panagiotis Xenopoulos, Silvia Muoz-Descalzo, Xinghua Lou, and Anna-Katerina Hadjantonakis   11. Quantitative Evaluation of Stem Cell Grafting in the Central Nervous System of Mice by In Vivo Bioluminescence Imaging and Post-Mortem Multi-Color Histological Analysis             Kristien Reekmans, Nathalie De Vocht, Jelle Praet, Debbie Le Blon, Chlo Hoornaert, Jasmijn Daans, Annemie Van der Linden, Zwi Berneman, and Peter Ponsaerts   12. Micro-CT Technique for Three Dimensional Visualization of Human Stem Cells             Andrea Farini, ChiaraVilla, Marzia Belicchi, Mirella Meregalli, and Yvan Torrente   13. Non-Invasive Multimodal Imaging of Stem Cell Transplants in the Brain Using Bioluminescence Imaging and Magnetic Resonance Imaging             Annette Tennstaedt, Markus Aswendt, Joanna Adamczak, and Mathias Hoehn   14. Magnetic Resonance Imaging and Tracking of Stem Cells             Hossein Nejadnik, Rostislav Castillo, and Heike E. Daldrup-Link   15. Whole Body MRI Followed by Fluorescent Microscopy Analysis for Detection of Stem Cells Labeled with Superparamagnetic Iron Oxide (SPIO) Nanoparticles and DiI Following Intracardiac Injection, Skeletal Muscle Transplantation, or Systemic Delivery             Boris Odintsov, Ju Lan Chun, and Suzanne E. Berry   16. Molecular Imaging and Tracking Stem Cells in Neurosciences             Toma Spiriev, Nora Sandu, and Bernhard Schaller   17. Bioluminescence Imaging of Human Embryonic Stem Cells-Derived Endothelial Cells for Treatment of Myocardial Infarction             Weijun Su, Liang Leng, Zhongchao Han, Zuoxiang He, and Zongjin Li