Bioluminescent Imaging: Methods and Protocols (Methods in Molecular Biology)

Bioluminescent Imaging: Methods and Protocols distills a wide range of techniques that use bioluminescence imaging as a tool for visualizing and tracking various biological processes. Covering diverse fields such as cellular and molecular biology, oncology, neurology, infectious diseases, immunology, and others, the detailed chapters of this volume are arranged by topic and describe practical procedures and applications of different bioluminescent reporters, from photoproteins (Aequorin) to bacterial luciferases as well as other secreted (such as Gaussia) and non-secreted luciferases (such as Firefly). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips for troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols aims to provide diverse and comprehensive techniques to researchers interested in implementing bioluminescence-based imaging in their laboratory, regardless of their previous level of experience with such methodologies. 1. Bioluminescence Imaging: Basics and Practical Limitations             Christian E. Badr  Part I: In Vitro Bioassays  2. Extraction and Quantification of Adenosine Triphosphate in Mammalian Tissues and Cells             Junji Chida and Hiroshi Kido  3. Neuronal Network Imaging in Acute Slices Using Ca2+ Sensitive Bioluminescent Reporter             Ludovic Tricoire and Bertrand Lambolez  4. Gaussia Luciferase-Based Mycoplasma Detection Assay in Mammalian Cell Culture             M. Hannah Degeling,  M. Sarah S. Bovenberg, Marie Tannous, and Bakhos A. Tannous  Part II: Imaging Molecular and Cellular Events  5. Split Gaussia Luciferase for Imaging Ligand-Receptor Binding             Kathryn E. Luker and Gary D. Luker  6. Video-Rate Bioluminescence Imaging of Protein Secretion from a Living Cell             Takahiro Suzuki and Satoshi Inouye  7. Bioluminescence Reporter Gene-Based Detection of MicroRNAs             Hae Young Ko, Young Sik Lee, and Soonhag Kim  8. Monitoring of Transcriptional Dynamics of HIF and NFB Activities             Miguel A.S. Cavadas and Alex Cheong  9. Real-Time Bioluminescent Tracking of Cellular Population Dynamics             Dan Close, Tingting Xu, Steven Ripp, and Gary Sayler  10. Fabrication of Bioluminescent Capsules and Live-Cell Imaging             Sung Bae Kim  Part III: Imaging of Disease (Pathogenesis)  11. The Bioluminescent Imaging of Spontaneously Occurring Tumors in Immunocompetent ODD-Luciferase Bearing Transgenic Mice             Scott J. Goldman and Shengkan Jin  12. Blood-Based Assay with Secreted Gaussia Luciferase to Monitor Tumor Metastasis             Hiroshi Yamashita, Dan T. Nguyen, and Euiheon Chung  13. Bioluminescence Imaging of Fungal Biofilm Development in Live Animals             Greetje Vande Velde, Sona Kucharikova, Patrick Van Dijck, and Uwe Himmelreich  14. Bioluminescent Imaging of Bacteria During Mouse Infection             Jonathan M. Warawa and Matthew B. Lawrenz  Part IV: Therapeutic Imaging  15. Cell-Based Bioluminescence Screening Assays             Romain J. Amante and Christian E. Badr  16. Bioluminescence-Based Monitoring of Virus Vector-Mediated Gene Transfer in Mice             Casey A. Maguire 17. Simultaneous In Vivo Monitoring of Regulatory and Effector T Lymphocytes Using Secreted Gaussia Luciferase, Firefly Luciferase, and Secreted Alkaline Phosphatase             Grant K. Lewandrowski, Ciara N. Magee, Marwan Mounayar, Bakhos A. Tannous, and Jamil Azzi  Part V: Emerging Technologies 18. Multiplex Functional Bioluminescent Reporters Using Gaussia Luciferase Fused to Epitope Tags in an Immunobinding Assay             Sjoerd van Rijn, Thomas Wrdinger, and Jonas Nilsson 19. Non-Invasive In Vivo Monitoring of Extracellular Vesicles             Charles P. Lai, Bakhos A. Tannous, and Xandra O. Breakefield 20. In Vitro and In Vivo Demonstrations of Fluorescence by Unbound Excitation from Luminescence (FUEL)             Joe Dragavon, Abdessalem Rekiki, Ioanna Theodorou, Chelsea Samson, Samantha Blazquez, Kelly L. Rogers, Rgis Tournebize, and Spencer Shorte