Cystic Fibrosis: Diagnosis and Protocols, Volume II: Methods and Resources to Understand Cystic Fibrosis (Methods in Molecular Biology)

Despite the many milestones in cystic fibrosis (CF) research, progress toward curing the disease has been slow, and it is increasingly difficult to grasp and use the already wide and still growing range of diverse methods currently employed to study CF so as to understand it in its multidisciplinary nature.  Cystic Fibrosis: Diagnosis and Protocols aims to provide the CF research community and related researchers with a very wide range of high-quality experimental tools, as an easy way to grasp and use classical and novel methods applied to cystic fibrosis.  Volume II: Methods and Resources to Understand Cystic Fibrosis focuses on pathophysiology, Omics approaches, and a variety of key resources recently made available for CF research.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Comprehensive and practical, Cystic Fibrosis: Diagnosis and Protocols will provide readers with optimal working tools to address pressing questions in the best technical way, while helping all of us, as a research and clinical community, to move faster hand-in-hand toward unravelling the secrets of this challenging disorder and cure it. Part I: Pathophysiology of Cystic Fibrosis   1. Introduction to Part I: Overview of Approaches to Study Cystic Fibrosis Pathophysiology             Mark T. Clunes and Richard C. Boucher   2. Imaging CFTR Protein Localization in Cultured Cells and Tissues             Silvia M. Kreda and Martina Gentzsch   3. CFTR Regulation of Epithelial Sodium Channel             Yawar J. Qadri, Estelle Cormet-Boyaka, Dale J. Benos, and Bakhrom K. Berdiev   4. Methods for Evaluating Inflammation in Cystic Fibrosis             Assem G. Ziady and Pamela B. Davis   5. Methods for ASL Measurements and Mucus Transport Rates in Cell Cultures             Erin N. Worthington and Robert Tarran   6. Measurement of Fluid Secretion from Intact Airway Submucosal Glands             Jeffrey J. Wine, Nam Soo Joo, Jae Young Choi, Hyungju Cho, Mauri E. Krouse, Jin V. Wu, Monal Khansaheb, Toshiya Irokawa, Juan Ianowski, John W. Hanrahan, Alan W. Cuthbert, and Kim V. Tran   7. Measurements of Intracellular Calcium Signals in Polarized Primary Cultures of Normal and Cystic Fibrosis Human Airway Epithelia             Carla M.P. Ribeiro   8. Identification and Quantification of Mucin Expression             Kristina A. Thomsson and Gunnar C. Hansson   9. Methods to Classify Bacterial Pathogens in Cystic Fibrosis             Thomas Bjarnsholt, Xiaohui Chen Nielsen, Ulla Johansen, Lena Nrgaard, and Niels Hiby   10. Approaches to Study Differentiation and Repair of Human Airway Epithelial Cells             Sophie Crespin, Marc Bacchetta, Song Huang, Tecla Dudez, Ludovic Wiszniewski, and Marc Chanson   Part II: Omic Approaches to Study Cystic Fibrosis   11. Introduction to Part II: Omics in the Biology of Cystic Fibrosis             William E. Balch   12. Microarray mRNA Expression Profiling to Study Cystic Fibrosis             Shyam Ramachandran, Luka A. Clarke, Todd E. Scheetz, Margarida D. Amaral, and Paul B. McCray, Jr.   13. Quantitative Differential Proteomics of Cystic Fibrosis Cell Models by SILAC (Stable Isotope Labelling in Cell Culture)             Ida Chiara Guerrera, Mario Ollero, Diane-Lore Vieu, and Aleksander Edelman   14. Application of Mass Spectrometry to Study Proteomics and Interactomics in Cystic Fibrosis             William E. Balch and John R. Yates III   15. Functional Genomics Assays to Study CFTR Traffic and ENaC Function             Joana Almaa, Shehrazade Dahimne, Nicole Appel, Christian Conrad, Karl Kunzelmann, Rainer Pepperkok, and Margarida D. Amaral   16. New Lipidomic Approaches in Cystic Fibrosis             Mario Ollero, Ida Chiara Guerrera, Giuseppe Astarita, Daniele Piomelli, and Aleksander Edelman   Part III: Resources   17. Introduction to Part III: Resources for CFTR Research             Margarida D. Amaral   18. Primary Epithelial Cell Models for Cystic Fibrosis Research             Scott H. Randell, M. Leslie Fulcher, Wanda O'Neal, and John C. Olsen   19. Comparative Biology of Cystic Fibrosis Animal Models             John T. Fisher, Yulong Zhang, and John F. Engelhardt   20. CFTR Folding Consortium: Methods Available for Studies of CFTR Folding and Correction             Kathryn W. Peters, Tsukasa Okiyoneda, William E. Balch, Ineke Braakman, Jeffrey L. Brodsky, William B. Guggino, Christopher M. Penland, Harvey B. Pollard, Eric J. Sorscher, William R. Skach, Philip J. Thomas, Gergely L. Lukacs, and Raymond A. Frizzell   21. Evaluation of the Disease Liability of CFTR Variants             Patrick R. Sosnay, Carlo Castellani, Mary Corey, Ruslan Dorfman, Julian Zielenski, Rachel Karchin, Christopher M. Penland, and Garry R. Cutting