Description
In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling. These include methods and protocols that are based on several mechanisms including type II and IIS restriction enzymes, single stranded annealing, sequence overlap, and recombination. With additional chapters on software programs that are suitable for primer design, a feature crucial for the functionality of the described methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, DNA Cloning and Assembly Methods seeks to provide scientist with a valuable and useful resource for wet lab researchers within life sciences. 1. BioBrick Assembly Standards and Techniques and Associated Software Tools Gunvor Rkke, Eirin Korvald, Jarle Pahr, Ove ys, and Rahmi Lale 2. Plasmid Construction by SLIC or Sequence and Ligation Independent Cloning Ryan E. Hill, Julian J. Eaton-Rye 3. Quick and Clean Cloning Frank Thieme and Sylvestre Marillonnet 4. Hierarchical Ligation-Independent Assembly of PCR Fragments Jonathan L. Schmid-Burgk, Zhen Xie, and Yaakov Benenson 5. USER-Derived Cloning Methods and Their Primer Design Bo Salomonsen, Uffe H Mortensen, Barbara A Halkier 6. Application of the Restriction-Free (RF) Cloning for Multi-Components Assembly Yoav Peleg and Tamar Unger 7. A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) Platform Ariel Erijman, Julia M. Shifman, and Yoav Peleg 8. Circular Polymerase Extension Cloning Jiayuan Quan and Jingdong Tian 9. Golden Gate Cloning Carola Engler and Sylvestre Marillonnet 10. Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy Alejandro Sarrion-Perdigones, Jorge Palaci, Antonio Granell, Diego Orzaez 11. FX cloning: A Simple and Robust High-Throughput Cloning Method for Protein Expression Eric R. Geertsma 12. Minimum GC-rich Sequences for Overlap Extension PCR and Primer Annealing Mikiko Nakamura, Ayako Suzuki, Hisashi Hoshida, and Rinji Akada 13. Simple Cloning and DNA Assembly in Escherichia coli by Prolonged Overlap Extension PCR Chun You and Y.-H. Percival Zhang 14. Combinatorial Assembly of Clone Libraries Using Site-Specific Recombination Vanessa E. Wall, Leslie A. Garvey, Jennifer L. Mehalko, Lauren V. Procter, and Dominic Esposito 15. Application of In-Fusion(TM) Cloning for the Parallel Construction of Escherichia coli Expression Vectors Louise E. Bird, Heather Rada, John Flanagan, Jonathan M. Diprose, Robert J.C. Gilbert, Raymond J. Owens 16. SeamLess Ligation Cloning Extract(SLiCE)Cloning Method Yongwei Zhang, Uwe Werling, and Winfried Edelmann 17. j5 DNA Assembly Design Automation Nathan J. Hillson 18. FASTPCR Software for PCR, in silico PCR, and Oligonucleotide Assembly and Analysis Ruslan Kalendar, David Lee, and Alan H. Schulman
