Description
In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, In Situ Hybridization Protocols, Fourth Edition seeks to aid scientists in the further discovery of new RNA species and uncovering of their cellular functions. 1. Fixation/Permeabilization Procedure for mRNA in situ Hybridization of Zebrafish Whole-mount Oocytes, Embryos, and Larvae Ricardo Fuentes and Juan Fernndez 2. MicroRNA In Situ Hybridization on Whole Mount Preimplantation Embryos Karen Goossens, Luc Peelman, and Ann Van Soom 3. Whole-mount in situ Hybridization (WISH) Optimized for Gene Expression Analysis in Mouse Embryos and Embryoid Bodies Eleni Dakou, Nele Vanbekbergen, Sara Corradi, Caroline R. Kemp, Erik Willems, and Luc Leyns 4. Whole Mount in Situ Hybridization using DIG-labeled Probes in Planarian Agnieszka Rybak-Wolf and Jordi Solana 5. In Situ Hybridization on Whole-Mount Zebrafish Embryos and Young Larvae Bernard Thisse and Christine Thisse 6. LNA-based in Situ Hybridization Detection of mRNAs in Embryos Diana K. Darnell and Parker B. Antin 7. Chromogen Detection of microRNA in Frozen Clinical Tissue Samples using LNA(TM) Probe Technology Boye Schnack Nielsen, Trine Mller, and Kim Holmstrm 8. MicroRNA In Situ Hybridisation in Tissue Microarrays Julia J. Turnock-Jones and John P.C. Le Quesne 9. Fluorescence in situ Hybridization for Detection of Small RNAs on Frozen Tissue Sections Asli Silahtaroglu 10. Sensitive and Specific in situ Hybridisation for Early Drug Discovery Pernille A. Usher, Elisabeth D. Galsgaard, Kimberly Kruse, Jishu Wang, Berit O. Krogh, Jette Mandelbaum, and Kasper Almholt 11. Zinc Based Fixation for High Sensitivity in situ Hybridization: A Non-Radioactive Colorimetric Method for the Detection of Rare Transcripts on Tissue Sections Electra Stylianopoulou, George Skavdis and Maria Grigoriou 12. Dual-color Ultrasensitive Bright-field RNA in situ Hybridization with RNAscope Hongwei Wang, Nan Su, Li-Chong Wang, Xingyong Wu, Son Bui, Allissa Nielson, Hong-Thuy Vo, Yuling Luo, and Xiao-Jun Ma 13. Fully Automated Fluorescence-based 4-color Multiplex Assay for Co-detection of microRNA and Protein Biomarkers in Clinical Tissue Specimens Lorenzo F. Sempere 14. Multiplexed miRNA Fluorescence in situ Hybridization for Formalin-Fixed Paraffin-Embedded Tissues Neil Renwick, Pavol Cekan, Claudia Bognanni, and Thomas Tuschl 15. Simultaneous Detection of Nuclear and Cytoplasmic RNA Variants Utilizing Stellaris RNA Fluorescence in situ Hybridization in Adherent Cells Sally R. Coassin, Arturo V. Orjalo, Jr., Sheila J. Semaan, and Hans E. Johansson 16. Quantitative Ultrasensitive Bright-field RNA in situ Hybridization with RNAscope Hongwei Wang, Nan Su, Li-Chong Wang, Xingyong Wu, Son Bui, Allissa Nielson, Hong-Thuy Vo, Yuling Luo, and Xiao-Jun Ma 17. Identification of Low Expressing Transcripts of the NPY Receptors’ Family in the Murine Lingual Epithelia Sergei Zolotukhin 18. In Situ Hybridization for Fungal Ribosomal RNA Sequences in Paraffin-Embedded Tissues Using Biotin-Labeled Locked Nucleic Acid Probes Kathleen T. Montone 19. In situ Hybridization Freeze-assisted Punches (IFAP): Technique for Liquid-based Tissue Extraction from Thin Slide-mounted Sections for DNA Methylation Analysis Lawrence S. Own and Paresh D. Patel 20. miRNA Detection at Single Cell Resolution Using Microfluidic LNA flow-FISH Meiye Wu, Matthew E. Piccini, and Anup K. Singh 21. PNA-based Fluorescence in situ Hybridization for Identification of Bacteria in Clinical Samples Mustafa Fazli, Thomas Bjarnsholt, Niels Hiby, Michael Givskov, and Tim Tolker-Nielsen
