Description
This volume aims to bring together a variety of protocols useful for DNA-based typing of blood cell antigens. Protocols range from simple approaches with low technical complexity to highly sophisticated modern developments. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Molecular Typing of Blood Cell Antigens summarizes contributions from leading scientist in the field DNA typing for blood cell antigens. 1. Validation Of Genotyping Protocols for Diagnostic Use Paul Metcalfe 2. High Resolution Melting Analysis Of Single Nucleotide Polymorphisms Carol M. Bruzzone And Clifford J. Steer 3. High-Speed Droplet-Allele-Specific Polymerase Chain Reaction for Genotyping Of Single Nucleotide Polymorphisms Kazuyuki Matsuda And Takayuki Honda 4. Blood Grouping Based On Pcr Methods and Agarose Gel Eletrophoresis Ana Maria Sell And Jeane Eliete Laguila Visentainer 5. Parallel Donor Genotyping For 46 Selected Blood Group and 4 Human Platelet Antigens Using High-Throughput Maldi-Tof Mass Spectrometry Stefan Meyer, Nadine Trost, Beat M. Frey, And Christoph Gassner 6. Pcr With Sequence-Specific Primers for Typing of Diallelic Blood Groups Gabriele Rink, Erwin A. Scharberg, and Peter Bugert 7. High-Resolution Melting Analysis for Genotyping Duffy Blood Group Antigens Ewa Lukasik, Kazimiera Wasniowska, Magdalena Grodecka, Edyta Majorczyk, and Marcin Czerwinski 8. Molecular Rhd-Rhce Analysis by Multiplex Pcr of Short Fluorescent Fragments Yann Fichou and Claude Frec 9. Microarrays in Blood Group Genotyping Stephanie A. Boccoz, Galle Le Goff, Loc J. Blum, and Christophe A. Marquette 10. Next Generation Sequencing for Antenatal Prediction of Kel1 Blood Group Status Klaus Rieneck, Frederik Banch Clausen, and Morten Hanefeld Dziegiel 11. 454-Sequencing(TM) For The Kel, Jr And Lan Blood Groups Carola Wieckhusen and Peter Bugert 12. Non-Invasive Prenatal Blood Group Genotyping Andrea Doescher and Thomas H. Mller 13. Genotyping Of Human Platelet Antigens By Beadchip Microarray Technology Gerald Bertrand and Fabiana Conti 14. Sequence-Based Typing For Platelet Alloantigens Shun-Chung Pai and Liang-In Lin 15. Miniaturized Technology For Dna Typing: Cassette Pcr Dammika P. Manage and Linda M. Pilarski 16. Geno- And Phenotyping Of Human Neutrophil Antigens Angelika Reil and Jrgen Bux 17. Allelic Discrimination by Taqman-Pcr For Genotyping of Human Neutrophil Antigens Rudi Steffensen, John Baech, and Kaspar R. Nielsen 18. Novel Approaches And Technologies in Molecular Hla Typing Paul P. J. Dunn 19. Luminex-Based Methods in High Resolution Hla Typing Manuela Testi and Marco Andreani 20. In Silico Hla Typing Using Standard Rna-Seq Sequence Reads Sebastian Boegel Jelle Scholtalbers, Martin Lwer, Ugur Sahin, and John C. Castle
