Description
RNA Interference: Challenges and Therapeutic Opportunities provides readers with recent advances in siRNA design, delivery, targeting and methods to minimize siRNA’s unwanted effects. Preclinical and clinical use of synthetic siRNAs, the roles of miRNAs in cancer and the promise of extracellular miRNAs for diagnosis are also covered in this meticulous collection, along with novel methods for identifying endogenous siRNAs and the annotation of small RNA transcriptomes. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.Comprehensive and cutting-edge, RNA Interference: Challenges and Therapeutic Opportunities will aid researchers, clinicians, teachers and biotechnologists interested in the power of RNA-based therapies. 1. RNA Interference: Mechanisms, Technical Challenges, and Therapeutic Opportunities Mouldy Sioud 2. Nanostructured RNAs for RNA Interference Yuko Nakashima, Naoko Abe, Yoshihiro Ito, and Hiroshi Abe 3. One Long Oligonucleotide or Two Short Oligonucleotides Based shRNA Construction and Expression Xue-jun Wang and Sheng-qi Wang 4. PLGA Microspheres Encapsulating siRNA Giuseppe De Rosa and Giuseppina Salzano 5. Magnetic Nanoparticle and Magnetic Field Assisted siRNA Delivery In Vitro Olga Mykhaylyk, Yolanda Sanchez-Antequera, Dialechti Vlaskou, Maria Belen Cerda, Mehrdad Bokharaei, Edelburga Hammerschmid, Martina Anton, and Christian Plank 6. Cytoplasmic Delivery of siRNAs to Monocytes and Dendritic Cells Via Electroporation Mouldy Sioud 7. Delivery of siRNAs to Cancer Cells Via Bacteria Omar Ahmed, Andrea Krhn, and Hermann Lage 8. Microwell Array-Mediated Delivery of Lipoplexes Containing Nucleic Acids for Enhanced Therapeutic Efficacy Yun Wu, Daniel Gallego-Perez, and L. James Lee 9. Modulating the Tumor Microenvironment with RNA Interference as a Cancer Treatment Strategy Karin Zins, Mouldy Sioud, Seyedhossein Aharinejad, Trevor Lucas, and Dietmar Abraham 10. Targeted In Vivo Delivery of siRNA and an Endosome-Releasing Agent to Hepatocytes Magdolna G. Sebestyn, So C. Wong, Vladimir Trubetskoy, David L. Lewis, and Christine I. Wooddell 11. Cell-Internalization SELEX: Method for Identifying Cell-Internalizing RNA Aptamers for Delivering siRNAs to Target Cells William H. Thiel, Kristina W. Thiel, Katie S. Flenker, Tom Bair, Adam J. Dupuy, James O. McNamara II, Francis J. Miller, and Paloma H. Giangrande 12. Strategies for siRNA Navigation to Desired Cells Mouldy Sioud 13. Analysis of RNA Interference-Mediated Hepatic Gene Silencing Following Murine Hydrodynamic Injection of Guanidinopropyl-Modified siRNAs Maximilian C.R. Buffb, Stefan Bernhardt, Musa D. Marimani, Abdullah Ely, Joachim W. Engels, and Patrick Arbuthnot 14. Therapy of Respiratory Viral Infections with Intranasal siRNAs Sailen Barik and Patrick Lu 15. Engineering Therapeutic Cancer Vaccines that Activate Anti-Tumor Immunity Per Ole Iversen and Mouldy Sioud 16. Immunosuppressive Factor Blockade in Dendritic Cells Via siRNAs Results in Objective Clinical Responses Mouldy Sioud, Anne Mobergslien, and Stein Sbe-Larssen 17. Targeting bcr-abl Transcripts with siRNAs in an Imatinib-Resistant Chronic Myeloid Leukemia Patient: Challenges and Future Directions Michael Koldehoff 18. A Facile Method for Interfering with Off-Target Silencing Mediated by the Sense Strand Anne Mobergslien and Mouldy Sioud 19. Overcoming the Challenges of siRNA Activation of Innate Immunity: Design Better Therapeutic siRNAs Mouldy Sioud 20. Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs Jia Han Deng, Peter Deng, Shi-Lung Lin, and Shao-Yao Ying 21. In Silico Identification of Novel Endo-siRNAs Andrew Schuster, Grant W Hennig, Nicole Ortogero, Dickson Luong, and Wei Yan 22. Computer-Assisted Annotation of Small RNA Transcriptomes Nicole Ortogero, Grant W. Hennig, Dickson Luong, and Wei Yan 23. miR-CATCH: MicroRNA Capture Affinity Technology Sebastian Vencken, Tidi Hassan, Noel G. McElvaney, Stephen G.J. Smith, and Catherine M. Greene 24. Roles of MicroRNAs in Cancers and Development Shigeru Takasaki 25. Targeting MicroRNAs to Withstand Cancer Metastasis Valentina Profumo, Valentina Doldi, Paolo Gandellini, and Nadia Zaffaroni 26. Urinary MicroRNAs as a New Class of Non-Invasive Biomarkers in Oncology, Nephrology, and Cardiology Hana Mlcochova, Renata Hezova, Albano C. Meli, and Ondrej Slaby 27. Magnetic Bead-Based Isolation of Exosomes Morten P. Oksvold, Axel Neurauter, and Ketil W. Pedersen 28. Modified 2′-Ribose Small RNAs Function as Toll-Like Receptor-7/8 Antagonists Mouldy Sioud
